老挝黄金赌场白洋（Yang Bai）课题组和储成才（Chengcai Chu）课题组的合作研究发现NRT1.1B与水稻根系微生物群组成和氮素利用有关。 相关论文于2019年发表在《Nature Biotechnology》杂志上。

研究人员应用16S核糖体RNA基因谱分析来表征在田间生长的68个籼稻和27个粳稻品种的根微生物群。发现籼稻和粳稻招募形成不同根微生物群。值得注意的是,与粳稻相比，籼稻根系细菌类群多样化程度更高，其根系富集更多与氮代谢相关的微生物种类。利用基因的方法,该课题组人员还发现NRT1.1B作为水稻的一种硝酸盐转运体和传感器，可以富集更多与氮循环相关的微生物种类。宏基因组测序揭示了在NRT1.1B突变体水稻显著影响水稻根系微生物组尤其是与氮循环相关的微生物组。该研究团队还从籼稻、粳稻根系微生物群中分离出1079种纯细菌菌株和特异富集菌群，并发现籼稻富集菌群与粳稻富集菌群相比，能够更好的改善有机氮条件下的水稻生长和氮素利用。该研究表明的植物基因型与根系微生物群之间的联系将会更好的指导今后提高氮素利用效率的育种策略。

据介绍，籼稻品种在氮肥利用效率方面通常表现出优于粳稻品种。

附：英文原文

Title: NRT1.1B is associated with root microbiota composition and nitrogen use in field-grown rice

Author: Jingying Zhang, Yong-Xin Liu, Na Zhang, Bin Hu, Tao Jin, Haoran Xu, Yuan Qin, Pengxu Yan, Xiaoning Zhang, Xiaoxuan Guo, Jing Hui, Shouyun Cao, Xin Wang, Chao Wang, Hui Wang, Baoyuan Qu, Guangyi Fan, Lixing Yuan, Ruben Garrido-Oter, Chengcai Chu, Yang Bai

Issue&Volume: Volume 37 Issue 6, June 2019

Abstract: Nitrogen-use efficiency of indica varieties of rice is superior to that of japonica varieties. We apply 16S ribosomal RNA gene profiling to characterize root microbiota of 68 indica and 27 japonica varieties grown in the field. We find that indica and japonica recruit distinct root microbiota. Notably, indica-enriched bacterial taxa are more diverse, and contain more genera with nitrogen metabolism functions, than japonica-enriched taxa. Using genetic approaches, we provide evidence that NRT1.1B, a rice nitrate transporter and sensor, is associated with the recruitment of a large proportion of indica-enriched bacteria. Metagenomic sequencing reveals that the ammonification process is less abundant in the root microbiome of the nrt1.1b mutant. We isolated 1,079 pure bacterial isolates from indica and japonica roots and derived synthetic communities (SynComs). Inoculation of IR24, an indica variety, with an indica-enriched SynCom improved rice growth in organic nitrogen conditions compared with a japonica-enriched SynCom. The links between plant genotype and root microbiota membership established in this study will inform breeding strategies to improve nitrogen use in crops.

DOI: 10.1038/s41587-019-0104-4

Source:https://www.nature.com/articles/s41587-019-0104-4