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粘膜愈合过程中B细胞扩张阻碍粘膜-上皮的再生交流
作者:小柯机器人 发布时间:2022/12/5 21:54:10

瑞典卡罗林斯卡学院Eduardo J. Villablanca小组发现,粘膜愈合过程中B细胞扩张阻碍粘膜-上皮的再生交流。2022年12月2日,《免疫》杂志在线发表了这项成果。

为了深入了解粘膜愈合的过程,研究人员纵向研究了肠道损伤和再生过程中的免疫细胞组成。B细胞是愈合的结肠中的主要细胞类型,单细胞RNA测序(scRNA-seq)显示,在实验性粘膜愈合期间,IFN诱导的B细胞亚群扩张,主要位于受损区域,并与结肠炎严重程度相关。来自上皮和基质的scRNA-seq与空间转录组学和多重免疫染色相结合,表明B细胞在粘膜愈合期间减少了基质和上皮细胞之间的相互作用。

活化的B细胞破坏了类器官存活所需的上皮细胞和基质细胞之间的交流。因此,B细胞在损伤期间的扩张损害了粘膜愈合所需的上皮-基质细胞的相互作用,这对治疗IBD有影响。

据介绍,治疗性促进肠道再生具有很大的前景,但确定影响组织再生的细胞机制仍然是一个未解决的挑战。

附:英文原文

Title: B cell expansion hinders the stroma-epithelium regenerative cross talk during mucosal healing

Author: Annika Frede, Paulo Czarnewski, Gustavo Monasterio, Kumar P. Tripathi, David A. Bejarano, Ricardo O. Ramirez Flores, Chiara Sorini, Ludvig Larsson, Xinxin Luo, Laura Geerlings, Claudio Novella-Rausell, Chiara Zagami, Raoul Kuiper, Rodrigo A. Morales, Francisca Castillo, Matthew Hunt, Livia Lacerda Mariano, Yue O.O. Hu, Camilla Engblom, Ana-Maria Lennon-Duménil, Romy Mittenzwei, Astrid M. Westendorf, Nadine Hvelmeyer, Joakim Lundeberg, Julio Saez-Rodriguez, Andreas Schlitzer, Srustidhar Das, Eduardo J. Villablanca

Issue&Volume: 2022-12-02

Abstract: Therapeutic promotion of intestinal regeneration holds great promise, but defining the cellular mechanisms that influence tissue regeneration remains an unmet challenge. To gain insight into the process of mucosal healing, we longitudinally examined the immune cell composition during intestinal damage and regeneration. B cells were the dominant cell type in the healing colon, and single-cell RNA sequencing (scRNA-seq) revealed expansion of an IFN-induced B cell subset during experimental mucosal healing that predominantly located in damaged areas and associated with colitis severity. B cell depletion accelerated recovery upon injury, decreased epithelial ulceration, and enhanced gene expression programs associated with tissue remodeling. scRNA-seq from the epithelial and stromal compartments combined with spatial transcriptomics and multiplex immunostaining showed that B cells decreased interactions between stromal and epithelial cells during mucosal healing. Activated B cells disrupted the epithelial-stromal cross talk required for organoid survival. Thus, B cell expansion during injury impairs epithelial-stromal cell interactions required for mucosal healing, with implications for the treatment of IBD.

DOI: 10.1016/j.immuni.2022.11.002

Source: https://www.cell.com/immunity/fulltext/S1074-7613(22)00592-1

期刊信息

Immunity:《免疫》,创刊于1994年。隶属于细胞出版社,最新IF:21.522
官方网址:https://www.cell.com/immunity/home
投稿链接:https://www.editorialmanager.com/immunity/default.aspx

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