武汉大学Xiang Zhou等研究人员合作发现，基于MOF的循环核酸提取方法揭示肝癌中的无细胞RNA特征。这一研究成果于2024年1月13日在线发表在国际学术期刊《国家科学评论》上。

研究人员开发了一种基于金属有机框架（MOF）材料包合核酸的可重复、高效的血清/血浆中不同类型无细胞核酸（含无细胞RNA（cfRNA）和病毒RNA）的分离技术，与最广泛使用的研究试剂盒方法相比，该技术大大提高了分离效率，并能保持RNA的完整性。

重要的是，用MOF方法提取的cfRNA质量约为试剂盒方法的10倍，而且MOF方法分离出的不同类型RNA是试剂盒方法的三倍多。研究人员描述了肝癌患者血清中cfRNA的全转录组图谱特征，并利用这种MOF方法鉴定出了包含6个cfRNA的cfRNA特征，诊断肝癌的效率很高（独立验证队列中的AUC= 0.905）。因此，这种新的MOF分离技术将推动液体活检领域的发展，并有可能诊断肝癌。

据了解，cfRNA可用于评估各种人体器官的健康状况、状态和表型，是一种潜在的生物标记物，可用于无创诊断多种疾病。然而，由于cfRNA丰度低且不稳定，目前还缺乏高效、无偏差的cfRNA分离技术。

附：英文原文

Title: Method for the extraction of circulating nucleic acids based on MOF reveals cell-free RNA signatures in liver cancer

Author: Sun, Yuqing, Yu, Haixin, Han, Shaoqing, Ran, Ruoxi, Yang, Ying, Tang, Yongling, Wang, Yuhao, Zhang, Wenhao, Tang, Heng, Fu, Boqiao, Fu, Boshi, Weng, Xiaocheng, Liu, Song-Mei, Deng, Hexiang, Peng, Shuang, Zhou, Xiang

Issue&Volume: 2024-01-13

Abstract: Cell-free RNA (cfRNA) allows assessment of health, status, and phenotype of a variety of human organs and is a potential biomarker to non-invasively diagnose numerous diseases. Nevertheless, there is a lack of highly efficient and bias-free cfRNA isolation technologies due to the low abundance and instability of cfRNA. Here, we developed a reproducible and high-efficiency isolation technology for different types of cell-free nucleic acids (containing cfRNA and viral RNA) in serum/plasma based on the inclusion of nucleic acids by metal-organic framework (MOF) materials, which greatly improved the isolation efficiency and was able to preserve RNA integrity compared with the most widely used research kit method. Importantly, the quality of cfRNA extracted by the MOF method is about 10-fold that of the kit method, and the MOF method isolates more than three times as many different RNA types as the kit method. The Whole transcriptome mapping characteristics of cfRNA in serum from patients with liver cancer was described and a cfRNA signature with 6 cfRNAs was identified to diagnose liver cancer with high diagnostic efficiency (AUC = 0.905 in the independent validation cohort) using this MOF method. Thus, this new MOF isolation technique will advance the field of liquid biopsy, with the potential to diagnose liver cancer.

DOI: 10.1093/nsr/nwae022

Source: https://dx.doi.org/10.1093/nsr/nwae022