研究人员揭示了柑橘油腺形成的分子框架。利用基因图谱和基因组编辑，研究人员证明了这一过程需要叶片锯齿的关键调控因子Late MERISTEM IDENTITY1（LMI1）。LMI1启动子中一个保守的GCC 盒元件招募DORNROSCHEN-like（DRNL）进行转录激活。DRNL-LMI1级联触发MYC5激活，促进油腺的发育和精油的生物合成。
Title: Molecular regulation of oil gland development and biosynthesis of essential oils in Citrus spp.
Author: Hongxing Wang, Jie Ren, Shiyun Zhou, Yaoyuan Duan, Chenqiao Zhu, Chuanwu Chen, Ziyan Liu, Qingyou Zheng, Shu Xiang, Zongzhou Xie, Xia Wang, Lijun Chai, Junli Ye, Qiang Xu, Wenwu Guo, Xiuxin Deng, Fei Zhang
Abstract: Secretory structures in terrestrial plants serve as reservoirs for a variety of secondary metabolites. Among these, the secretory cavity of the Rutaceae family is notable for containing essential oils with a wide range of applications. However, the molecular basis underlying secretory cavity development is unknown. Here, we reveal a molecular framework for Citrus oil gland formation. Using genetic mapping and genome editing, we demonstrated that this process requires LATE MERISTEM IDENTITY1 (LMI1), a key regulator of leaf serration. A conserved GCC box element of the LMI1 promoter recruits DORNROSCHEN-like (DRNL) for transcriptional activation. This DRNL-LMI1 cascade triggers MYC5 activation, facilitating the development of oil glands and the biosynthesis of essential oils. Our findings spotlight cis-regulatory divergence within leaf shape genes, propelling novel functional tissue formation.