哈尔滨工业大学贺强团队报道了旋转F_oF₁-ATP-ATP合成酶驱动的烧瓶状戊聚糖胶体马达与ATP合成和储存。相关研究成果发表在2024年4月10日出版的《美国化学会杂志》。

研究人员报道了叶绿体衍生的旋转F_oF₁-ATP酶马达驱动的瓶状戊聚糖胶体马达（FPCM）的分级组装，该马达具有合成、储存和触发释放生物能量货币ATP的能力。这些流线型和亚微米大小的中空烧瓶状戊聚糖胶体马达，是通过将基于软模板的水热聚合与含有旋转F_oF₁-ATP-ATP酶马达的，叶绿体衍生的蛋白脂质体的真空输注相结合来制备的。

通过注射酸性缓冲溶液在蛋白脂质体上产生质子原动力，促进F_oF₁-ATP-ATP酶马达的旋转，以驱动FPCMs的自推进运动，伴随着内部ATP的合成和储存。这些旋转F_oF₁-ATP-ATP酶马达驱动的FPCMs，通过沿着外部环境的质子梯度（负趋化性）从其颈部开口，迁移到其圆形底部而表现出趋化行为。

这种旋转生物分子马达驱动的具有ATP合成和按需释放的烧瓶状戊聚糖胶体马达，使其成为工程新型智能纳米载体以积极调节细胞代谢的有前途的候选者。

附：英文原文

Title: Rotary FoF1-ATP Synthase-Driven Flasklike Pentosan Colloidal Motors with ATP Synthesis and Storage

Author: Yue Li, Jun Liu, Yingjie Wu, Qiang He

Issue&Volume: April 10, 2024

Abstract: We report the hierarchical assembly of a chloroplast-derived rotary FoF1-ATPase motor-propelled flasklike pentosan colloidal motor (FPCM) with the ability of the synthesis, storage, and triggered release of biological energy currency ATP. These streamlined and submicrometer-sized hollow flasklike pentosan colloidal motors are prepared by combining a soft-template-based hydrothermal polymerization with a vacuum infusion of chloroplast-derived proteoliposomes containing rotary FoF1-ATPase motors. The generation of proton motive force across the proteoliposomes by injecting an acidic buffer solution promotes the rotation of FoF1-ATPase motors to drive the self-propelled motion of FPCMs, accompanying the inner ATP synthesis and storage. These rotary FoF1-ATPase motor-powered FPCMs exhibit a chemotactic behavior by migrating from their neck opening to their round bottom along a proton gradient of the external environment (negative chemotaxis). Such rotary biomolecular motor-driven flasklike pentosan colloidal motors with ATP synthesis and on-demand release make them promising candidates for engineering novel intelligent nanocarriers to actively regulate cellular metabolism.

DOI: 10.1021/jacs.4c00334

Source: https://pubs.acs.org/doi/abs/10.1021/jacs.4c00334