济南大学孔祥正团队报道了具有LCST和UCST行为的非常规荧光多响应聚乙烯亚胺的合成、表征和生物应用。相关研究成果发表在2024年4月16日出版的《中国高分子科学杂志》。

近年来，非芳香族荧光和多响应材料因其固有的荧光发射和可控的相变而备受关注。然而，它们的荧光性质和相变之间潜在的相互作用仍不清楚。

该文中，研究人员通过使聚乙烯亚胺（PEI）与N-异丙基丙烯酰胺（NIPAM）反应，合成了一系列无催化剂的氮杂迈克尔加成基聚乙烯亚胺（RFPEI）材料。对所得的RFPEI进行了全面表征，并在水中表现出双相变行为（LCST和UCST），可以通过调节其组成或pH等外部因素进行微调。值得注意的是，在紫外线照射（365nm）下，RFPEI表现出强荧光发射。

研究人员进一步研究了NIPAM接枝率、聚合物浓度和pH对RFPEI水溶液的LCST/UCST和荧光性能的影响。此外，研究人员展示了RFPEI作为生理细胞成像、微量检测和阿霉素控释的多功能工具的巨大潜力。

该研究提出了一类新的刺激响应荧光材料，在生物医学领域具有很好的应用前景。

附：英文原文

Title: Unconventional Fluorescent and Multi-responsive Polyethyleneimine with LCST and UCST Behavior: Synthesis, Characterization and Biological Applications

Author: Feng-Ming Yin, Li-Li Wu, Shu-Sheng Li, Xiao-Na Pan, Xiao-Li Zhu, Xu-Bao Jiang, Xiang Zheng Kong

Issue&Volume: 2024-04-16

Abstract: Non-aromatic fluorescent and multi-responsive materials, exhibiting inherent fluorescence emission and controlled phase change, have garnered significant attention in recent years. However, the underlying interaction between their fluorescent properties and phase transition remains unclear. In this study, we synthesized a series of catalyst-free aza-Michael addition-based polyethyleneimine (RFPEI) materials by reacting polyethyleneimine (PEI) with N-isopropyl acrylamide (NIPAM). The resulting RFPEI was comprehensively characterized, and demonstrated dual-phase transition behavior (LCST and UCST) in water, which could be finely tuned by adjusting its composition or external factors such as pH. Notably, upon UV irradiation (365 nm), RFPEI exhibited strong fluorescence emission. We further investigated the effects of NIPAM grafting percentage to PEI, polymer concentration, and pH on the LCST/UCST and fluorescent properties of RFPEI aqueous solutions. Moreover, we showcased the great potential of RFPEI as a versatile tool for physiological cell imaging, trace detection, and controlled release of doxorubicin. Our study presents a novel class of stimuli-responsive fluorescent materials with promising applications in the field of biomedicine.

DOI: 10.1007/s10118-024-3120-x

Source: https://www.cjps.org/en/article/doi/10.1007/s10118-024-3120-x/