研究人员从野外白纹伊蚊（Aedes albopictus）的肠道中发现了一种名为Rosenbergiellasp. YN46（Rosenbergiella_YN46）的细菌，它能使蚊子对登革热和寨卡病毒感染产生抵抗力。将1.6×103个菌落形成单位（CFU）的Rosenbergiella_YN46接种到A. albopictus体内可有效防止病毒感染。从机理上讲，这种细菌会分泌葡萄糖脱氢酶（RyGDH），酸化喂食蚊子的肠腔，导致黄病毒包膜蛋白发生不可逆的构象变化，从而阻止病毒进入细胞。
在半田间条件下，Rosenbergiella_YN46在田间蚊子中表现出有效的跨株传播，从而阻止了登革热病毒通过新出现的成蚊传播。Rosenbergiella_YN46在登革热低流行地区蚊子中的流行率（52.9%至91.7%）高于在登革热流行地区蚊子中的流行率（0%至6.7%）。Rosenbergiella_YN46可能为黄病毒的生物控制提供有效而安全的线索。
研究人员表示，蚊子肠道中的共生微生物群在决定虫媒病毒的传播能力方面起着复杂的作用。
附：英文原文
Title: A naturally isolated symbiotic bacterium suppresses flavivirus transmission by Aedes mosquitoes
Author: Liming Zhang, Daxi Wang, Peibo Shi, Juzhen Li, Jichen Niu, Jielong Chen, Gang Wang, Linjuan Wu, Lu Chen, Zhenxing Yang, Susheng Li, Jinxin Meng, Fangchao Ruan, Yuwen He, Hailong Zhao, Zirui Ren, Yibaina Wang, Yang Liu, Xiaolu Shi, Yunfu Wang, Qiyong Liu, Junhua Li, Penghua Wang, Jinglin Wang, Yibin Zhu, Gong Cheng
Issue&Volume: 2024-04-19
Abstract: The commensal microbiota of the mosquito gut plays a complex role in determining the vector competence for arboviruses. In this study, we identified a bacterium from the gut of field Aedes albopictus mosquitoes named Rosenbergiella sp. YN46 (Rosenbergiella_YN46) that rendered mosquitoes refractory to infection with dengue and Zika viruses. Inoculation of 1.6 × 103 colony forming units (CFUs) of Rosenbergiella_YN46 into A. albopictus mosquitoes effectively prevents viral infection. Mechanistically, this bacterium secretes glucose dehydrogenase (RyGDH), which acidifies the gut lumen of fed mosquitoes, causing irreversible conformational changes in the flavivirus envelope protein that prevent viral entry into cells. In semifield conditions, Rosenbergiella_YN46 exhibits effective transstadial transmission in field mosquitoes, which blocks transmission of dengue virus by newly emerged adult mosquitoes. The prevalence of Rosenbergiella_YN46 is greater in mosquitoes from low-dengue areas (52.9 to ~91.7%) than in those from dengue-endemic regions (0 to ~6.7%). Rosenbergiella_YN46 may offer an effective and safe lead for flavivirus biocontrol.
DOI: adn9524
Source: https://www.science.org/doi/10.1126/science.adn9524