老挝黄金赌场于乐谦 、郭靖涛、王晓琰和中国农业大学魏育蕾研究组，合作报道了人类原肠胚的三维重建。相关论文发表在2024年4月23日出版的《细胞》杂志上。

研究人员对卡内基阶段（CS）8细胞人类胚胎的62个横切面上的38562个位点，进行了高分辨率空间转录组学分析。根据这一空间转录组数据集，研究人员构建了CS8胚胎的三维模型，其中根据基因表达模式和位置信息，沿着胚胎的前-后轴、内-外轴和背-腹轴确定了一系列细胞亚型。

研究进一步描述了胚胎和胚胎外组织的系谱轨迹和相关调控因子，以及在胚胎发育过程中支撑系谱发展和组织形态的，信号中心和信号活动区域化信息。总之，这项研究为人类胚胎的原肠胚形成和后原肠胚发育提供了见解。

据介绍，由于缺乏自然胚胎参考数据集，特别是那些在原肠胚形成等关键阶段的空间信息数据集，对人类早期发育的研究受到阻碍。

附：英文原文

Title: 3D reconstruction of a gastrulating human embryo

Author: Zhenyu Xiao, Lina Cui, Yang Yuan, Nannan He, Xinwei Xie, Sirui Lin, Xiaolong Yang, Xin Zhang, Peifu Shi, Zhifeng Wei, Yang Li, Hongmei Wang, Xiaoyan Wang, Yulei Wei, Jingtao Guo, Leqian Yu

Issue&Volume: 2024-04-23

Abstract: Progress in understanding early human development has been impeded by the scarcityof reference datasets from natural embryos, particularly those with spatial informationduring crucial stages like gastrulation. We conducted high-resolution spatial transcriptomicsprofiling on 38,562 spots from 62 transverse sections of an intact Carnegie stage(CS) 8 human embryo. From this spatial transcriptomic dataset, we constructed a 3Dmodel of the CS8 embryo, in which a range of cell subtypes are identified, based ongene expression patterns and positional register, along the anterior-posterior, medial-lateral,and dorsal-ventral axis in the embryo. We further characterized the lineage trajectoriesof embryonic and extra-embryonic tissues and associated regulons and the regionalizationof signaling centers and signaling activities that underpin lineage progression andtissue patterning during gastrulation. Collectively, the findings of this study provideinsights into gastrulation and post-gastrulation development of the human embryo.

DOI: 10.1016/j.cell.2024.03.041

Source: https://www.cell.com/cell/abstract/S0092-8674(24)00357-X